A Two-step Procedure for Adventitious Shoot Regeneration from in vitro-derived Lingonberry Leaves: Shoot Induction with TDZ and Shoot Elongation Using Zeatin
نویسندگان
چکیده
منابع مشابه
Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot.
Relatively high percentages of adventitious shoot regeneration have consistently been obtained from leaves of some apricot cultivars. For the cultivar 'Helena', explants from the proliferation medium were more reactive than those from the elongation medium. The best results were obtained with thidiazuron (TDZ). When 6-benzylamino-purine (BAP) was used instead of TDZ, the regeneration percentage...
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A protocol has been developed for the direct induction of adventitious shoots from leaf explants of in vitro raised Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. The Murashige and Skoog (MS) media supplemented with N–benzyladenine (BA), kinetin (KIN), and thidiazuron (TDZ) were used in regeneration experiments. BA at 0.5 mg L concentration was chosen as the optimal treat...
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In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...
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Different explants of Crocus sativus L. such as corm, style, ovary, petal, anther and leaf were cultured on LS and MS media supplemented with NAA and BAP or 2,4-D plus BAP at different combinations. Shoot and corm induction occurred in addition to the formation of other organs. Efficient plantlet regeneration with strong roots occurred at 1 mg 1-I of both NAA and BAP. An increase in 2,4-D l...
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An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...
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ژورنال
عنوان ژورنال: HortScience
سال: 2005
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.40.1.189